Anti-Bacterial Action of Zinc Oxide Nanoparticles against Skin Bacterial Infection
DOI:
https://doi.org/10.61704/pr.655Keywords:
Zno, Nanoparticles , Anti-Bacterial Activity, AntibioticsAbstract
It has been observed that metal nanoparticles containing oxides of Znic Oxide, copper, silver, nickel, iron furthermore other elements exhibit antibacterial qualities. The antibacterial action of zinc oxide nanoparticles against a variety of species of pathogenic bacteria, including Klebsiella pneumonia, Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa is examined in this study. Using dilutions of [200, 100, 50, 25, and 12.5 µg/mL], the disk diffusion test existed employed to judge the antibacterial action of Zno NPs and conclude the MIC, MBC of each isolate. Through arise in inhibition area diameter that is straight proportionate to a rise in nanoparticle concentration, Zno NPs exhibits potent wide-ranging antibacterial action against tested bacteria, even surpassing the activity of certain medications. When compared to the chosen antibiotics, P.vulgaris appeared to be the least sensitive isolate, while Pseudomonas aeruginosa demonstrated great sensitivity even at 12.5 µg/mL. P.aeruginosa, with the MBC ranging since 25 to 200 µg/mL and the MIC of Zno NPs ranging from 12. µg/mL to 50 µg/mL.It is greatly recommended consuming Zno NPs as a profitable alternate antibacterial cause particularly in handling ectopic pathogenic outbreaks deprived of attractive danger of evolving resistant bacterial isolates with antibiotics.
References
Agarwal, H., Menon, S., Kumar, S. V., & Rajeshkumar, S. (2018). Mechanistic study on antibacterial action of zinc oxide nanoparticles synthesized using green route. Chemico-Biological Interactions, 286, 60–70. https://doi.org/10.1016/j.cbi.2018.03.008
Alothman, A. A., & Albaqami, M. D. (2020). Nano-sized Cu(II) and Zn(II) complexes and their use as a precursor for synthesis of CuO and ZnO nanoparticles: A study on their sonochemical synthesis, characterization, and DNA-binding/cleavage, anticancer, and antimicrobial activities. Applied Organometallic Chemistry, 34(10), Article e5827. https://doi.org/10.1002/aoc.5827
Clinical and Laboratory Standards Institute. (2025). Performance standards for antimicrobial susceptibility testing (35th ed., CLSI standard M100).
El-Fallal, A. A., Elfayoumy, R. A., & El-Zahed, M. M. (2023). Antibacterial activity of biosynthesized zinc oxide nanoparticles using Kombucha extract. SN Applied Sciences, 5(12), Article 332. https://doi.org/10.1007/s42452-023-05546-x
Hameed, A. S. H., Karthikeyan, C., Ahamed, A. P., Thajuddin, N., Alharbi, N. S., Alharbi, S. A., & Ravi, G. (2016). In vitro antibacterial activity of ZnO and Nd doped ZnO nanoparticles against ESBL producing Escherichia coli and Klebsiella pneumoniae. Scientific Reports, 6, Article 24312. https://doi.org/10.1038/srep24312
Hoseinzadeh, E., Alikhani, M.-Y., Samarghandi, M.-R., & Shirzad-Siboni, M. (2014). Antimicrobial potential of synthesized zinc oxide nanoparticles against gram positive and gram negative bacteria. Desalination and Water Treatment, 52(25–27), 4969–4976. https://doi.org/10.1080/19443994.2013.810356
Jiang, J., Pi, J., & Cai, J. (2018). The advancing of zinc oxide nanoparticles for biomedical applications. Bioinorganic Chemistry and Applications, 2018(1), Article 1062562. https://doi.org/10.1155/2018/1062562
Karekar, S. E., Bhanvase, B. A., Sonawane, S. H., Deosarkar, M. P., Pinjari, D. V., & Pandit, A. B. (2015). Synthesis of zinc molybdate and zinc phosphomolybdate nanopigments by an ultrasound assisted route: Advantage over conventional method. Chemical Engineering and Processing: Process Intensification, 87, 51–59. https://doi.org/10.1016/j.cep.2014.11.010
Kołodziejczak-Radzimska, A., & Jesionowski, T. (2014). Zinc oxide—From synthesis to application: A review. Materials, 7(4), 2833–2881. https://doi.org/10.3390/ma7042833
Liao, C., Jin, Y., Li, Y., & Tjong, S. C. (2020). Interactions of zinc oxide nanostructures with mammalian cells: Cytotoxicity and photocatalytic toxicity. International Journal of Molecular Sciences, 21(17), Article 6305. https://doi.org/10.3390/ijms21176305
Pachaiappan, R., Rajendran, S., Show, P. L., Manavalan, K., & Naushad, M. (2021). Metal/metal oxide nanocomposites for bactericidal effect: A review. Chemosphere, 272, Article 128607. https://doi.org/10.1016/j.chemosphere.2020.128607
Padilla-Cruz, A. L., Garza-Cervantes, J. A., Vasto-Anzaldo, X. G., García-Rivas, G., León-Buitimea, A., & Morones-Ramírez, J. R. (2021). Synthesis and design of Ag–Fe bimetallic nanoparticles as antimicrobial synergistic combination therapies against clinically relevant pathogens. Scientific Reports, 11, Article 5351. https://doi.org/10.1038/s41598-021-84768-8
Punjabi, K., Mehta, S., Chavan, R., Chitalia, V., Deogharkar, D., & Deshpande, S. (2018). Efficiency of biosynthesized silver and zinc nanoparticles against multi-drug resistant pathogens. Frontiers in Microbiology, 9, Article 2207. https://doi.org/10.3389/fmicb.2018.02207
Qi, K., Cheng, B., Yu, J., & Ho, W. (2017). Review on the improvement of the photocatalytic and antibacterial activities of ZnO. Journal of Alloys and Compounds, 727, 792–820. https://doi.org/10.1016/j.jallcom.2017.08.142
Raheem, H. Q., Hussein, E. F., Rasheed, A. H., & Imran, N. K. (2022). Antibacterial action of silver nanoparticles against Staphylococcus aureus isolated from wound infection. Research Journal of Pharmacy and Technology, 15(6), 2413–2416. https://doi.org/10.52711/0974-360X.2022.00401
Rasha, E., Alkhulaifi, M. M., AlOthman, M., Khalid, I., Doaa, E., Alaa, K., Awad, M. A., & Abdalla, M. (2021). Effects of zinc oxide nanoparticles synthesized using Aspergillus niger on carbapenem-resistant Klebsiella pneumonia in vitro and in vivo. Frontiers in Cellular and Infection Microbiology, 11, Article 748739. https://doi.org/10.3389/fcimb.2021.748739
Shanmugam, R., Munusamy, T., Jayakodi, S., Al-Ghanim, K. A., Nicoletti, M., Sachivkina, N., & Govindarajan, M. (2023). Probiotic-bacteria (Lactobacillus fermentum)-wrapped zinc oxide nanoparticles: Biosynthesis, characterization, and antibacterial activity. Fermentation, 9(5), Article 413. https://doi.org/10.3390/fermentation9050413
Siddiqi, K. S., Rahman, A., Tajuddin, & Husen, A. (2018). Properties of zinc oxide nanoparticles and their activity against microbes. Nanoscale Research Letters, 13, Article 141. https://doi.org/10.1186/s11671-018-2532-3
Tiwari, V., Mishra, N., Gadani, K., Solanki, P. S., Shah, N. A., & Tiwari, M. (2018). Mechanism of anti-bacterial activity of zinc oxide nanoparticle against carbapenem-resistant Acinetobacter baumannii. Frontiers in Microbiology, 9, Article 1218. https://doi.org/10.3389/fmicb.2018.01218
Tripathy, S., & Sahu, S. K. (2019). FtsZ inhibitors as a new genera of antibacterial agents. Bioorganic Chemistry, 91, Article 103169. https://doi.org/10.1016/j.bioorg.2019.103169
Sonnenberg, G. F., Fouser, L. A., & Artis, D. (2011). Border patrol: Regulation of immunity, inflammation and tissue homeostasis at barrier surfaces by IL-22. Nature Immunology, 12(5), 383–390. https://doi.org/10.1038/ni.2025
Zanet, V., Vidic, J., Auger, S., Vizzini, P., Lippe, G., Iacumin, L., Comi, G., & Manzano, M. (2019). Activity evaluation of pure and doped zinc oxide nanoparticles against bacterial pathogens and Saccharomyces cerevisiae. Journal of Applied Microbiology, 127(5), 1391–1402. https://doi.org/10.1111/jam.14407
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Copyright © 2025 by the authors. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). You may not alter or transform this work in any way without permission from the authors. Non-commercial use, distribution, and copying are permitted, provided that appropriate credit is given to the authors and Al-Hadba University.
